Hplc analysis of monosaccharides of some home grown wood species

The paper presents a qualitative and quantitative analysis of monosaccharides in prevalent home-grown hardwood species. The aim of this investigation was to determine the possibility of using the wood residue of examined species for a production of sugar. Oak, chestnut, maple, beech, elm, walnut, cherry, ash and alder wood samples were analysed. Monosaccharides were identified by HPLC - High Performance Liquid Chromatography. Monosaccharicle solution was prepared by hydrolysis with sulphuric acid. Monosaccharides were quantitatively determined using BIOTRONIC LC 5001 apparatus combined with SI-IIMADZU C-R6A CHROMATOPAC integrator. Results of investigation show that glucose and xylose are present in significant amounts in all examined wood samples, whereas galactose, mannose and rhamnose were also identified in all wool samples but in very small amounts. The obtained results show that the HPLC method applied here is advantageous as compared with the usual gravimetric and volumetric procedures for the analysis of monosaccharides in hardwoods.