Effect of Extraction Technique on Composition of Volatile Constituents of Oleoresin from Pinus Brutia Ten.

In this study, volatile constituents of oleoresin from Pinus brutia Ten. were extracted by solid phase microextraction (SPME) and hydrodistillation. Gas chromatography-mass spectroscopy (GC/MS) was performed to determine volatile constituents of turpentine oil (obtained by hydrodistillation) and extract from oleoresin (obtained by SPME). Eleven volatile compounds constituted the total of turpentine oil obtained by hydrodistillation, whereas SPME extract contained 32 volatile compounds. Three distinctive volatile constituents in the turpentine oil were α-pinene (49.12 %), β-pinene (19.12 %) and Δ3-carene (15.33 %). They were represented in the amount of 44.35 %, 18.59 % and 14.58 % in the SPME extract. The volatile constituents of turpentine oil were monoterpenes and sesquiterpenes. Monoterpenes, sesquiterpenes, terpene oxides, alcohols, aldehydes, esters and ethers were identified as main groups in the SPME extract by GC/MS. The results of chromatographic analysis showed that solid phase microextraction was a more effective extraction technique than hydrodistillation for isolating volatile constituents from oleoresin.